Biospecimen Repository and Processing Core

    

Directors: David Geller, MD, and Aatur Singhi, MD, PhD; Associate Director: Michael Nalesnik, MD; Core Manager: Kate Smith

The Biospecimen Repository and Processing Core provides a centralized location for investigators to request liver-disease-related tissues, cells, and TMAs. Requests for specimens are made via an online request form (see below). A review committee considers each request and makes a determination regarding distribution of specimens based on project relevance and feasibility. Those investigators who would like to request tissues must have up-to-date IACUC, IRB, and/or CITI certification and must be able to provide proof of certification.

The core is able to provide:

Fresh/frozen patient liver tissue

Available upon approval of committee

Searchable database forthcoming

Paraffin sections from various liver pathologies (malignant and non-malignant)

Available upon approval of committee

Searchable database forthcoming

Cell lines

 - HepG2
 - Hep3B
 - Snu398
 - Snu443
 - Huh7
 - Aml12
 - Huvec

Primary Cell Lines

 - Hepatocytes, human – available upon approval of committee.

 - Hepatocyte, mouse
 - Hepatocyte, rat
 - Non-parenchymal cell, mouse
 - Non-parenchymal cell, rat

Histology Services

 
 - H&E (Process & embed)
 - Recut H&E
 - Unstained blanks
 - Tunel Assay
 - Special Stains (Grocott, PAS, Gram, AFB, FiteM/N, etc.)
 - Immunoperoxidase (antibody provided-routinely run stain)
 - Double IHC stain
 - In situ hybridization
 - PD-L1 Antibody
 - FISH
 

Mouse Blood Services

Analysis services provided by CLB:

 - ALT
 - AST
 - BR direct
 - BR total
 - GGT
 - ALP
 - ALB
 

 

For the request form, please click here. Send completed request forms and all attachments to Kate Smith (kms386@pitt.edu)