Genomics and Systems Biology Core

  

Directors: Jianhua Luo, MD, PhD and Takis Benos, PhD

  

Associate Directors:  Yanping Yu, MD, PhD and Maria Chikina, PhD

Core Manager: Silvia (Shuchang) Liu, PhD


The GSBC has introduced both the Oxford Nanopore sequencer and a single cell machine:

Oxford Nanopore long-read sequencing
  • Whole genome sequencing for de novo assembly, structural variation, single nucleotide variation and resequencing analyses
  • Transcriptome sequencing for RNA isofrom and fusion transcript analyses using direct RNA sequencing and ligation sequencing
  • Targeted sequencing include amplicons, sequence capture and exome
  • Epigenetic sequencing including methylation, histone modification and non-coding RNA activity
  • Microbiome sequencing
10X Genomics single cell sequencing
  • Our center is equipped with ControllerTM from 10x Genomics, Inc. We provide collaboration on the analysis of 3’ barcoded and 5’ barcoded single-cell transcriptome sequencing to classify cell type on individual cell level. We also provide exome, copy number and ATAC sequencing for the chromosome structure analysis, all on the single-cell level. Single-cell classification provides high-resolution analysis on cell population. It is a new tool that enables us to understand organ development, cancer evolution and immune cell activation in individual cell level.  
 
The core is able to provide the following Genomics services:
  • U133 2.0 array 
  • R230 2.0 array 
  • R430 2.0 array 
  • CLARIOM array 
  • SNP 6.0 array 
  • CytoscanHD 
  • OncoScan array 
  • Human exon 1.0 ST array 
  • Expression arrays for porcine, bovine, drosophila, etc. 

 

Illumina sequencing

  • Library ready sequencing
  • Library prep from raw sample or RNA
  • Library prep for whole genome from raw sample or DNA
  • Library prep for exome from raw sample or DNA
  • Library prep for methylation from raw sample or DNA
  • Library prep for single cell sequencing with individual molecule barcoded

To request Genomics and/or Systems Biology services, please complete the online request form available by clicking here.